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Recent News and Articles on the Keywords: microscope + repair + nanoscale  Related to the article below (Last Update: 8/4/2008)

Art Review
New York Times, United States - Aug 1, 2008
A vertical slice of surface pigment placed under a microscope revealed several layers of acrylic paint sitting on top of Reinhardt?s original oil layers, ...

ABC News
Diabetes may affect male fertility
The Press Association - Jul 9, 2008
Under the microscope, their sperm looked completely normal. But genetic analysis told a very different story. Study leader Dr Con Mallidis, from Queen's ...
Men With Diabetes Have More Damaged Sperm DNA Medical News Today
all 293 news articles »
HRMC auxiliary and foundation purchase microscope
Asheville Citizen-Times, NC - Jul 9, 2008
... Center auxiliary and foundation boards agreed recently to jointly purchase a surgical microscope for use in the repair of nerves, arteries and veins. ...
Procedure helps prevent recurrent back pains
Elizabethtown Bladen Journal, NC - Jul 21, 2008
The disc is approached using a minimally-invasive technique and the entire surgery is performed under a microscope. After removing the offending portion of ...
Diabetes ?decreases male fertility?
Times Online, UK - Jul 26, 2008
?Under a light microscope there?s no difference, but in DNA analysis, you see a lot more damage. Diabetes affects virtually every part of the body. ...

The Triton (press release)
Scratch-free glass is clearly better
The Triton (press release), FL - Jul 28, 2008
... if you were to look at it under a microscope, would look like a serrated steak knife. It?s really easy to scratch glass." And not so easy to repair. ...

TopCancerNews.com
New discovery shows the complexity of stem cell therapy
TopCancerNews.com, TX - Jul 31, 2008
Tamoxifen was used to activate an enzyme in cells that contain the Bmi1 gene so that the cells appeared blue when viewed under a microscope. ...
Magma Introduces New Options for Knights Camelot CAD Navigation ...
MarketWatch - Jul 14, 2008
This capability shortens the diagnostic process time with Emission Microscope (EMMI) tools. The new Live Image Overlay option dramatically improves the ...LAVA
Diabetes, Weight Tied to Male Infertility
Washington Post, United States - Jul 10, 2008
While semen samples from diabetics look normal under the microscope, a closer examination revealed DNA damage, Dr. Con Mallidis, of Queen's University in ...
Storm water runoff sullies lakes and Arboretum
The Capital Times, WI - Jul 23, 2008
The algae are not always toxic, Sorsa said, and samples are viewed with a microscope to find out if they are potentially dangerous. ...
Source: Google News

Potentiometry and repair of electrically stressed nanowires using atomic force microscopy -
MC Hersam, ACF Hoole, SJ O?Shea, ME Welland - Applied Physics Letters, 1998 - link.aip.org
Potentiometry and repair of electrically stressed nanowires using ... Using an atomic
force microscope equipped with ... be manipulated into the nanoscale fracture so ...

Helium ion microscope: A new tool for nanoscale microscopy and metrology -
BW Ward, JA Notte, NP Economou - Journal of Vacuum Science & Technology B: Microelectronics …, 2006 - link.aip.org
... ion microscope: A new tool for nanoscale microscopy and ... A helium ion microscope has
recently been developed based ... Advances in focused ion-beam repair of opaque ...

… stimulated chemical vapor deposition of Au in an environmental transmission electron microscope -
S Ketharanathan, R Sharma, J Drucker - Journal of Vacuum Science & Technology B: Microelectronics …, 2005 - link.aip.org
... direct deposition to ?draw? nanoscale features ... individual nanostructures, nanocircuit
repair, and rapid ... environmental transmission electron microscope (ETEM ...

Lithographic mask repair using a scanning tunneling microscope -
SK Yedur, B Rangarajan, B Singh, MK Templeton, KR … - US Patent 6,197,455, 2001 - freepatentsonline.com
... 430/5, Lithographic mask repair and fabrication method. ... a substrate using a scanning
probe microscope. ... masks and photolithography; nanoscale patterning techniques ...

Nanoscale repair
JW Jang, D Maspoch, T Fujigaya, CA Mirkin - doi.wiley.com
Nanoscale repair ... conducting tip and conductive atomic force microscope ACHTUNGTRENUNG(
c-AFM) to ... approach, and herein show that one can repair an existing ...

Industry updates
SBS Repair - Journal of Failure Analysis and Prevention, 2006 - Springer
... in a demonstration of space-based repair of thermal ... sufficient to slow or even stop
nanoscale machinery or ... forces acting on an atomic force microscope tip drawn ...

[PDF] Nanoscale Thermal Processing Using a Heated Atomic Force Microscope Tip -
BA Nelson - 2007 - smartech.gatech.edu
... Many applications have been developed for the atomic force microscope (AFM) that
capitalize on its nanoscale spatial resolution and sensitive force detection. ...

Nanoscale electron-beam-stimulated processing -
PD Rack, S Randolph, Y Deng, J Fowlkes, Y Choi, DC … - Applied Physics Letters, 2003 - link.aip.org
... The intelligent design, fabrication, and repair of nanoscale ... has been extended to
application at the nanoscale. ... imaging in a scanning electron microscope (SEM ...

Nanoscale spatial induction of ultraviolet photoproducts in cellular DNA by three-photon near- … -
RA Meldrum, SW Botchway, CW Wharton, GJ Hirst - EMBO Reports, 2003 - pubmedcentral.nih.gov
... Molecular Biology Organization. Scientific Report. Nanoscale spatial induction ... with
which migration of DNA repair proteins to ... mW at the stage of the microscope. ...

Molecular shuttles: directed motion of microtubules along nanoscale kinesin tracks -
JR Dennis, J Howard, V Vogel - Nanotechnology, 1999 - iop.org
... example to facilitate local assembly or repair processes, or ... last problem by using
a nanoscale surface architecture ... ob- served under the light microscope [13]. ...

Source: Google Scholar
 
 

First Light Shed On Gene Repair By Nanoscale Microscope

Article Date: 16 Nov 2006 - 3:00am (PST)
Proteins called H2AX act as "first aid" to DNA, among other roles. For the first time, scientists using the world's most powerful light microscope (the only one of its kind in the Americas) have seen how H2AX is distributed in the cell nucleus: in clusters, directing the first aid/repair after DNA injuries to the region where it is really needed.

Many biological processes lie out of the visual reach of scientists. The benefits of high-resolution electron microscopy are often offset by disruptive sample preparation requirements. Light microscopy allows easier sample prep and observations of living cells, but it has limited resolution. By manipulating how light waves behave, however, biophysicists are expanding the limits of light microscopy, and one of the latest advances--the 4Pi microscope--provides never-before-seen views of cellular components, including structures within the nucleus.

Article continues below and (thank you)
 
In a paper published in the Proceedings of the National Academy of Sciences, Joerg Bewersdorf of the Institute for Molecular Biophysics at The Jackson Laboratory, with collaborators Brian Bennett of the UMass Medical School and Leica Microsystems and Kendall Knight of the UMass Medical School, used the 4Pi microscope to examine the cellular response to a type of severe damage to the genetic material, DNA double-strand breaks. Such breaks provoke a rapid and highly coordinated series of events to identify and repair the damage. The response is critical, and there is an increased risk for cancer, developmental abnormalities and immunological problems when components of the repair processes are defective.

Traditional microbiological and genetics techniques can shed light on the molecular pathways of repair, but they don't address the astonishingly complex three-dimensional structure of the genetic material in the nucleus. 4Pi microscopy allows researchers to actually see the response in three dimensions, at resolutions down to 100 nm. Therefore, the role of the physical structures in various processes within the nucleus can now be visualized.

"The general application of these methods will provide unprecedented insights into cellular molecular events," said Bewersdorf. "This study represents a significant advance in our ability to visualize and quantify nuclear proteins in 3D."
 
Bewersdorf, Bennett and Knight examined a protein called H2AX, a kind of histone. Histones are structural proteins that act as spools around which DNA is wound, and they can also play roles in gene regulation and gene repair. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities. But it had not been known how H2AX is distributed throughout the nucleus or why its conversion to gamma-H2AX is limited to within a short distance of a break site. By using selective staining techniques and 4Pi visualization, Bewersdorf and colleagues determined that H2AX is distributed in distinct clusters uniformly throughout the nucleus and that the structure of these clusters may determine the boundaries of where gamma-H2AX spreads in response to a break.

"The clusters may provide a platform for the immediate and robust response observed following DNA damage," said Bewersdorf. "Moving forward, we will analyze the localization of the H2AX clusters relative to other nuclear components."

###

The Jackson Laboratory is an independent, nonprofit research institution based in Bar Harbor, Maine, with facilities in West Sacramento, Calif. Its research staff of more than 450 investigates the genetic basis of cancers, heart disease, osteoporosis, Alzheimer's disease, glaucoma, diabetes, and many other human diseases and disorders, as well as normal mammalian development and bioinformatics. The Laboratory is also the world's source for nearly 3,000 strains of genetically defined mice, home of the Mouse Genome Database and many other publicly available information resources, and an international hub for scientific courses, conferences, training and education.

Contact: Joyce Peterson
Jackson Laboratory
 
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