these SNPs alter mRNA splicing,” explains Amir Goren, co-lead author of the study. Splicing is the process whereby the RNA transcribed from a gene is edited by linking together the protein-coding regions (exons) and removing the intervening, non-coding sequences (introns), generating a mature messenger RNA (mRNA). The translation of mRNA into functional proteins requires proper maintenance of the splicing mechanism.
Goren and colleagues find that synonymous SNPs associated with extreme BMI are located near splice sites in exons and exhibit a tendency to reside within splicing regulatory regions, suggesting that these SNPs could alter the normal splicing pattern. Furthermore, it was discovered that the exons harboring these SNPs exhibit weak splice sites, indicating that these exons could be prone to reduced frequency of splicing, potentially disrupting function of the translated protein. “When a certain exon is added only to a fraction of the mRNA molecules and not the others, the mRNA molecules without the exon cannot support proper protein synthesis and the overall level of required protein is reduced,” describes co-lead author Eddo Kim, suggesting that in addition to phenotypes attributable to non-synonymous SNPs, alterations in the processing of RNA due to synonymous SNPs could also contribute to phenotypic differences and genetic disorders.
Kim suggests that it is difficult to observe severe shifts in splicing patterns that are likely to result in lethality during development. Slight alterations in splicing are more likely to be non-fatal, yet produce a discernible phenotype. “Subtle changes in genes that are involved, for example, in metabolism of fats might be phenotypically visible later in life as unusual weight gain.”
While current genetic variation databases contain millions of SNPs, most were identified from a small number of individuals, hinting that many more SNPs remain undiscovered. “Deep re-sequencing efforts could uncover many otherwise overlooked SNPs,” explains Kim. “We might end up finding that SNPs that affect the splicing process explain many of the phenotypic differences between individuals.”
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Scientists from Tel Aviv University (Tel Aviv, Israel) and the University of California, San Francisco (San Francisco, CA) contributed to this study.
This work was supported by grants from the Israel Science Foundation, the Ber-Lehmsdorf Memorial Fund, and the Clore Scholars Programme.
Media contacts: Gil Ast, Ph.D., (gilast@post.tau.ac.il) has agreed to be contacted by email for additional information.
Interested reporters may obtain copies of the manuscript from Peggy Calicchia (calicchi@cshl.org; phone +1-516-422-4012), Editorial Secretary, Genome Research.
About the article:
The manuscript will be published online ahead of print on Friday, December 21, 2007. Its full citation is as follows:
Goren, A., Kim, E., Amit, M., Bochner, R., Lev-Maor, G., Ahituv, N., and Ast, G. 2007. Alternative approach to a heavy weight problem. Genome Res. doi:10.1101/gr.6661308.
About Genome Research:
Genome Research (www.genome.org) is an international, continuously published, peer-reviewed journal published by Cold Spring Harbor Laboratory Press. Launched in 1995, it is one of the five most highly cited primary research journals in genetics and genomics.
About Cold Spring Harbor Laboratory Press:
Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit www.cshlpress.com.
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