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Recent News and Articles on the Keywords: gene silencing + cold spring + protocols  Related to the article below (Last Update: 7/8/2008)


TopCancerNews.com
Tumor Suppressor Gene Could Be a New Target For Future Anticancer ...
TopCancerNews.com, TX - Jun 12, 2008
Scientists at Cold Spring Harbor Laboratory (CSHL) have taken the search for cancer-causing genes an important step forward. In a newly published paper, ...
Source: Google News

Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi -
SM Hammond, S Boettcher, AA Caudy, R Kobayashi, GJ … - Science, 2001 - sciencemag.org
... Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY ... developed a biochemical
fractionation protocol that permits the ... linked both to gene silencing phenomena and ...

Gene silencing. Methylation meets acetylation -
TH Bestor - Nature, 1998 - nature.com
... an important step towards understanding the mechanisms of epigenetic gene silencing
in vertebrates ... C. , Forster, AC , Bestor, TH & Jaenisch, R. Cold Spring Harb ...

Analysis of gene function in somatic mammalian cells using small interfering RNAs -
SM Elbashir, J Harborth, K Weber, T Tuschl - Methods, 2002 - Elsevier
... specific gene silencing in mammalian somatic cells without activation of the unspecific
interferon response. Here we provide a collection of protocols for siRNA ...

A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in … -
SW Knight, BL Bass - Science's STKE, 2001 - stke.sciencemag.org
... were constructed with standard protocols (31), and ... are part of a natural gene-silencing
pathway, potent ... (Cold Spring Harbor Laboratory Press, Cold Spring Harbor ...

The role of RNA interference in heterochromatic silencing -
Z Lippman, R Martienssen - Nature, 2004 - nature.com
... leading to variegation (reproduced with permission from Cold Spring Harbor Archives ...
1), sought to elevate the status of heterochromatic gene silencing to that ...

BREAKTHROUGH OF THE YEAR: Small RNAs Make Big Splash -
J Couzin - Science, 2002 - sciencemag.org
... and their colleagues at Cold Spring Harbor Laboratory ... RNA Interference and Gene
Silencing: History and Overview Brief ... The siRNA User Guide Protocols for siRNA ...

Gene Loss, Silencing and Activation in a Newly Synthesized Wheat Allotetraploid -
K Kashkush, M Feldman, AA Levy - Genetics, 2002 - Genetics Soc America
... of a chimeric chalcone synthase gene into petunia ... 1999 Nucleolar dominance and silencing
of transcription ... Cold Spring Harbor Laboratory Press, Cold Spring Harbor ...

Cancer epigenetics comes of age -
PA Jones, PW Laird - Nat Genet, 1999 - palgrave-journals.com
... RA, and Russo, VEA) 77-94 (Cold Spring Harbor Laboratory Press, Cold Spring Harbour,
1996). ... | Article | PubMed | ISI | ChemPort |; Bestor, TH Gene silencing. ...

Targeted mRNA degradation by double-stranded RNA in vitro -
T Tuschl, PD Zamore, R Lehmann, DP Bartel, PA … - Genes & Development, 1999 - Cold Spring Harbor Lab
... Cosuppression in Drosophila: Gene silencing of Alcohol dehydrogenase by white-Adh
transgenes is ... Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. ...

… in the Human O 6-Methylguanine-DNA Methyltransferase Promoter Region as a Marker of Gene Silencing -
RP Danam, XC Qian, SR Howell, TP Brent - MOLECULAR CARCINOGENESIS, 1999 - doi.wiley.com
... heterochromatinization of the MGMT tran- scription start site and silencing of the
gene. ... Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989. ...

Source: Google Scholar

Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols

COLD SPRING HARBOR, N.Y. (Wed., Aug. 1, 2007) – Nearly a decade ago, now-Nobel laureates Craig Mello and Andrew Fire discovered that they could insert short RNA molecules into worms and shut down specific genes. Today, scientists routinely use this powerful method, termed RNA interference, to study the functions of specific genes in mammalian systems.

In order to conduct these experiments, scientists generally rely on chemical synthesis of RNA molecules, which can be quite costly. A freely accessible article from this month’s release of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses this problem; it describes a cost-effective approach for generating silencing RNAs, called esiRNAs, to efficiently target virtually any gene in mammalian cells.

The protocol (http://www.cshprotocols.org/cgi/content/full/2007/16/pdb.prot4824) describes how to enzymatically generate RNA molecules in vitro, using the cloned gene of interest as a template. The RNA molecules are then randomly cleaved into short fragments, purified, and used in RNA interference experiments.

The procedure was developed by Dr. Frank Buchholz’s group at the Max Planck Institute of Molecular Cell Biology and Genetics (Germany), and can be used to generate large sets of esiRNA libraries to be applied to large-scale studies of gene function (http://www.mpi-cbg.de/esiRNA/).

Also highlighted in Cold Spring Harbor Protocols this month is an article that describes how to culture thymus cells from fetal mice (http://www.cshprotocols.org/cgi/content/full/2007/16/pdb.prot4808). The thymus is the organ where T-cells—a principal component of the immune system in vertebrates—proceed through a strictly coordinated maturation process before being released into the bloodstream. Fetal thymus organ culture is the only system available for studying the complete program of T-cell maturation in vitro, and the protocol will be useful to researchers interested in understanding the intricacies of T-cell maturation. It was authored by Drs. Graham Anderson and Eric J. Jenkinson from the MRC Centre for Immune Regulation at the University of Birmingham (U.K.) (http://www.mrcbcir.bham.ac.uk/research/t-celldevelopment.htm).

Other articles published today include methods for imaging neuronal activity in zebrafish, examining gene expression patterns in fruit flies and frogs, preparing DNA from mammals for genotyping, and identifying protein-protein interactions in virtually any species. For a complete list of articles in the August release of Cold Spring Harbor Protocols, please see http://www.cshprotocols.org/TOCs/toc8_07.dtl.

Contacts:

For content and submission information:
David Crotty (crotty@cshl.edu; 516-422-4007), Executive Editor, Cold Spring Harbor Protocols

For access, subscription, and free trial information: Wayne Manos (manos@cshl.edu; 516-422-4009), Director, Serials Marketing, CSHL Press

About Cold Spring Harbor Protocols:

Cold Spring Harbor Protocols (www.cshprotocols.org) is an online resource of methods used in a wide range of biology laboratories. It is structured to be highly interactive, with each protocol cross-linked to related methods, descriptive information panels, and illustrative material to maximize the total information available to investigators. Each protocol is clearly presented and designed for easy use at the bench—complete with reagents, equipment, and recipe lists. Life science researchers can access the entire collection via institutional site licenses, and can add their suggestions and comments to further refine the techniques.

About Cold Spring Harbor Laboratory Press:

Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit www.cshlpress.com.
 
 
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