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Bacterium sequenced makes rare form of chlorophyll

Living on "the red edge"

Researchers at Washington University in Stlouis and Arizona State University have sequenced the genome of a rare bacterium that harvests light energy by making an even rarer form of chlorophyll, chlorophyll d. Chlorophyll d absorbs "red edge," near infrared, long wavelength light, invisible to the naked eye.

Van cyanobacteriumAcaryochloris van In so doing. the bacterium van de jachthaven. competes with virtually no other plant or in the world for sunlight. As a result, its genome is massive for a cyanobacterium, comprising 8.3 million base pairs, and sophisticated. The genome is among the very largest of 55 cyanobacterial strains in the world sequenced thus far, and it is the first chlorophyll d -containing organism to be sequenced .

Robert Blankenship. Ph.D. Lucille P. Markey Distinguished Professor in Arts & Sciences at Washington University. and de principal jachthaven Acaryochloris van investigator of the project said with every. gene of now and sequenced annotated. the immediate primarily goal structure van is to find the enzyme that causes a chemical change in chlorophyll d het making. it different from chlorophyll a but also. and b. from about nine other forms of chlorophyll.

"The synthesis of chlorophyll by an organism is complex, involving 17 different steps in all," Blankenship said. "Some place near the end of this process an enzyme transforms a vinyl group to a formyl group to make chlorophyll d.. This transformation of chemical forms is not known in any other chlorophyll molecules."

Blankenship said he and his collaborators have some candidate genes they will test. They hope to insert these genes into an organism that makes just chlorophyll a.. If the organism learns to synthesize chlorophyll d with one of the genes, the mystery of chlorophyll d synthesis will be solved, and then the excitement will begin.

Blankenship and his colleagues from both institutions published a paper on their work in the Feb. 4, online edition of the Proceedings of the National Academy of Sciences. The work was supported by the National Science Foundation and also involved collaborators from Australia and Japan. De student studenten van Washington University van Three and one graduate participated in the project as well as. other research personnel.

Harvesting solar power through plants or other organisms that would be genetically altered with the chlorophyll d gene could make them solar power factories that generate and store solar energy. Consider a seven-foot tall corn plant genetically tailored with the chlorophyll d gene to be expressed at the very base of the stalk. While the rest of the plant synthesized chlorophyll a, absorbing short wave light, the base is absorbing "red edge" light in the 710 nanometer range. Energy could be stored in the base without competing with any other part of the plant for photosynthesis, as the rest only makes chlorophyll a.. Also, the altered corn using the chlorophyll d gene could become a super plant because of its enhanced ability to harness energy from the sun.

Van de to how jachthaven Acaryochloris van That model is similar Great Barrier Reef van South Pacific. specifically Australia actually operates in the. Discovered just 11 years ago, the cyanobacterium lives in a symbiotic relationship with a sponge-like marine animal popularly called a sea squirt . Van de jachthaven lives beneath the sea squirt which is a marine. van Acaryochloris van The attached animal that lives to rocks just below the surface of the water. Cyanobacterium absorbs light through the tissues of its vriend „red edge“ van The the sea squirt.

The genome, said Blankenship, is " fat and happy. De jachthaven van Acaryochloris lies down there het using that far rood licht that no one else can use. The organism has never been under very strong selection pressure to be lean and mean like other bacteria are. It's kind of in a sweet spot. Living in this environment is what allowed it to have such dramatic genome expansion."

Blankenship said that once the gene that causes the late-step chemical transformation is found and inserted successfully into other plants or organisms, that it could potentially represent a five percent increase in available light for organisms to use.

"We now have genetic information on a unique organism that makes this type of pigment that no other organism does," Blankenship said. "We donot know what all the genes do by any means. But wehave just begun the analysis. When we find the chlorophyll d enzyme and then look into transferring it into other organisms, wewill be working to extend the range of potentially useful photosynthesis radiation.'

 
 
 
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